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fungal cultures and enzymes, but particularly cell and plant culture Technically, shear refers to the relative motion of notional layers of liquid past one another due to a velocity gradient (or shear strain, dv/dy), and shear stress refers to the force per unit area acting tangentially to the surface of an object The forces acting on a cell suspended in a uid are the result of the dissipation of uid kinetic energy to the external surface of the cell The magnitude of these forces is a function of the uid viscosity and velocity gradient immediately surrounding a cell and is given by dv = = (18-73) dy In laminar ow, the shear stress can be calculated relatively easily (Prokop and Bajpai, 1992) However, the situation is more complex in the turbulent ow regime.

Glycerophospholipid (general structure)

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(7.9)

In this case the instantaneous velocity vector is the sum of the timeaveraged velocity and a randomly uctuating time-dependent velocity vector The intensity of turbulence is directly related to the magnitude of the uctuating velocity vector Therefore, a cell suspended in laminar ow will experience an average shear stress that is independent of time, while in turbulent ow it can experience high shear stresses due to turbulence intermittency (Baldyga and Bourne, 1995; Baldyga et al, 2001) These authors point out that the local rate of energy dissipation displays uctuations about its mean even under homogeneous ow conditions These are intermittent (rare) uctuations due to high energy events that are dif cult to measure directly but could have a vital role in understanding cell uid interactions Intermittency is especially important in cases where changes are irreversible and occur over long time frames (as is the case in cell culture systems).

N(t) 17 19 40 18 15 19 37 20

Consideration of intermittency also suggests that it is scale dependent, which may serve to undermine the conventional approach of using average energy dissipation rates for estimating cell damage or drop breakage in immiscible liquids In fact, this was clearly shown in the highly signi cant paper of Baldyga et al (2001), where difference in the steady-state Sauter mean drop diameter obtained at two different scales at equal mean energy dissipation rates could be explained by considering turbulence intermittency Almost equally abused is the Kolmogorov theory of isotropic turbulence (Kolmogorov, 1941a,b) According to this theory, kinetic energy at suf ciently large Reynolds numbers is imparted to the uid by the rotating impeller blade and is initially transported by eddies (the largest eddy size is determined by the size of the impeller blade) The kinetic energy in the large eddies is rapidly cascaded to smaller eddies in an isotropic manner (ie.

complementary mRNA (-)

Continuous mixing is used to mix ingredients continuously in a mixer in a single pass. The ingredient quantity to be mixed may vary in any range; however, unlike batch mixing, care must be taken to feed the mixer in a controlled fashion. Mixing in batch often leads to variation in the mixing quality, which can be controlled or almost eliminated by continuous mixers. Even though continuous mixing is gaining popularity, selection of continuous mixers is not as straightforward as batch, which can be accomplished by running trials. Continuous mixers are more compact than batch mixers. While discharging from the mixers, segregation can be reduced by positioning the discharge closer to packaging units or as an integral part of it. In continuous mixing, mixing has to be achieved in both the radial and axial (in the direction of conveying) directions.

, directional information is lost), until nally most of the energy imparted by the impeller is transferred to the uid via viscous dissipation of small eddies These eddies are characterized by a length scale called the Kolmogorov microscale (see Section 18-51) and are often referred to as the universal equilibrium range of wavenumbers (inverse of the eddy size), where the energy carried by the eddies is independent of viscosity The kinetic energy of turbulence is transferred from eddies to the cell surface to an extent determined by the relative size of eddies and the cells Thus if the eddy.

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