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transcription factor antibodies listed in Table 5.1 bene t from exposure to boiling sodium citrate for approximately 5 min. The other antibodies have needed no speci c unmasking. (f) Aspirate the permeabilization solution and wash the cells in PBSA. (g) Remove PBSA and apply standard ICC protocols or those provided by the supplier of the primary antibody. (h) Depending on whether bright eld or uorescence ICC has been performed cells can be counterstained with Toluidine Blue or DAPI to aid visualization of cell nuclei. Similarly, choice of mounting solution will depend on the use of glass slide or tissue culture plastic.

Some shapes are hard to draw using a mouse. For example, you could create the complex shape in Figure 27.22 by drawing each of the shaded areas as a separate, closed path. Or you could simply create a square, place four circles in front of it so that they overlap the edges of the square, and then choose Object Paths Make Compound Path a process that takes only a few seconds. That s what I did, in fact, to get the result shown in the right side of Figure 27.22. I converted the five closed paths on the left into a compound path by choosing Object Paths Make Compound Path to create the shape on the right. InDesign automatically applied the attributes of the original square path, which is the backmost path, to the resulting compound path. Notice that the four semicircular areas where the original shapes overlapped became holes after the shapes were converted to a compound path. That s only the beginning of what you can do with the Make Compound Path command. Mix in a little bit of your imagination and InDesign s other path-, graphic-, and text-manipulation features, and the possibilities become endless.

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Rijndael uses a second interpretation for the components in a byte x (x0, x1, . . . , x6, x7) [ Z2,8, namely, as the coef cients of a polynomial of degree 7 x(z) ; x0 z 7 x1 z 6 x6 z x0 $ x (x0 , x1 , . . . , x6 ; x7 ): The addition of bytes x y is according to the usual rules for the addition of polynomials, Rijndael refers to addition as EXOR rather than XOR. Associating a byte with a polynomial provides a way to de ne the multiplication; if x(z) ; x0 z7 x1 z6 x6 z x7 $ x (x0 , x1 , . . . , x6 , x7 ) y(z) ; y0 z 7 y1 z 6 y6 z y7 $ y (y0 , y1 , . . . , y6 , y7 ) then z(z) ; z0 z7 z1 z 6 z6 z z7 $ z (z0 , z1 , . . . , z6 , z7 ) ; x y,

Consider a computation on a distributed system that is st,arted by a special process called environment. This process start,s up the computation by sending messages t,o

Most browsers depict the default bullet as a small filled-in circle. In HTML, you can use any of three strategies to change the shape of your bullets. Dreamweaver supports all these strategies. Your choices are to Add an attribute to the <ul> or <li> tag to specify one of several other bullet shapes. Simulate an unordered list by using an image of the bullet. Use Cascading Style Sheets to define a new bullet shape.

Fig. 5-7. Accel grid current-to-beam current ratio as a function of the beamlet current for three values of the beam voltage.

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Illustrator can create a chart for a company s organization. Using the Rectangle tool and some effects, you can make a clean organization chart. To create an organization chart, whether it s for a business or simply a family tree, follow these steps:

Pressing Alt (Option) when using the Knife tool cuts in a straight line rather than a curved one. Pressing Shift constrains the straight line to a 45 angle when you also press Alt (Option). Remember that the Knife tool works on all paths that are under the existing path, selected or not.

in it. If you need to create several different sets of permissions, create a different folder for each.